In-Vitro Prevention of Glucose Induced Cataract by Second Generation Sulfonylurea: Glibenclamide. Goat eye lenses were divided into four groups. Group I lenses were incubated in artificial aqueous humor with glucose concentration 5.5 mM (normal control). Group II lenses were incubated with glucose concentration 55 mM. Group III and IV lenses incubated with glucose concentration 55 mM were incubated along with Glibenclamide 10 and 20 ng/ml and subjected to biochemical evaluation. Glucose induced opacification of goat lens began 8-10 hrs after incubation and was complete in 72-80 hrs. Cataractous lenses showed higher Na+, MDA, lower concentrations of protein content. Lenses treated with Glibenclamide showed higher protein content and prevented formation and progress of cataract by glucose, as evidenced by biochemical parameters. The anticataract activity of Glibenclamide may be because of the antioxidant and free radical scavenging activity, as evidenced by a decrease in MDA in treated lenses. Further in-vitro and in-vivo studies in various experimental models and long term clinical trials are required to validate the anticataract activity of Second Generation Sulfonylurea: Glibenclamide.
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